THE SMART TRICK OF 2-FDCK HCL THAT NOBODY IS DISCUSSING

The lab suggests fixation will trigger the dyes to leak in the cells. I believe the assumption is fixation could hurt cell membranes. I did look for a paper which describes fixation utilizing methanol or acetic acid, following staining which has a DCFDA by-product. DCFDA is definitely the reagent that stains ROS in the package ab113851, and I think